QIAcube

Overview

The QIAcube provides automated processing of QIAGEN spin columns. No change of purification chemistry is required. All steps in the purification procedure are fully automated and up to 12 samples can be processed per run.

The QIAcube is preinstalled with protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. The range of protocols available is continually expanding, and additional QIAGEN protocols can be downloaded free of charge.

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Scheduling

Most protocols require 1 to 1 ½ hours complete.

Appointments may be reserved by Pennington employees using the Outlook calendar. The QIAcube is listed in Outlook as R_GCF_QIAcube.

Detailed instructions for using the Outlook calendar to schedule an instrument (resource) may be found in the Scheduling Resources - Frequently Asked Questions.

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Cost

The cost for the QIAcube is $5.00 per run (1 to 12 samples).

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Training

Training is required before use of this instrument.

New users should contact GCF for training and reagent requirements.

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Protocols

ProcedureStarting materialQIAGEN kit
Purification of bisulfite converted DNA Bisulfite conversion reaction EpiTect Bisulfite Kit
Purification of plasmid DNA E. coli cultures QIAprep Spin Miniprep Kit
DNA cleanup Amplification reactions QIAquick PCR Purification Kit
MinElute PCR Purification Kit
Agarose gel slices QIAquick Gel Extraction Kit
Enzymatic reactions MinElute Reaction Cleanup Kit
QIAquick Nucleotide Removal Kit
Purification of genomic DNA Whole blood, body fluids, or buccal swabs QIAamp DNA Blood Mini Kit
Tissues QIAamp DNA Mini Kit
Stool QIAamp DNA Stool Mini Kit
Animal blood or cells, tissues, and rodent tails DNeasy Blood & Tissue Kit
Plant cells and tissues DNeasy Plant Mini Kit
Purification of bacterial DNA Biologcal fluids, swabs, or bacterial cultures QIAamp DNA Mini Kit
Purification of viral nucleic acids Plasma, serum, or cell-free body fluids QIAamp MinElute Virus Spin Kit
Purification of total RNA Animal cells and easy-to-lyse tissues RNeasy Plus Mini Kit
RNeasy Mini Kit
FFPE tissue sections RNeasy FFPE Kit
Fatty tissues RNeasy Lipid Tissue Mini Kit
Aqueous phase of tissue or cell homogenates treated with chloroform miRNeasy Mini Kit
Plant cells and tissues RNeasy Plant Mini Kit
Small amounts of tissues or small numbers of cells RNeasy Micro Kit
Purification of viral RNA Cell-free body fluids QIAamp Viral RNA Mini Kit
RNA cleanup RNA RNeasy MinElute Cleanup Kit
RNeasy Mini Kit
Purification of 6xHis-tagged proteins E. coli cultures or eukaryotic cells Ni-NTA Spin Kit
Depletion of serum proteins Serum or plasma Qproteome Albumin/IgG Depletion Kit
Qproteome Murine Albumin Depletion

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