The Genomics Core Facility offers the use of a NanoDrop Spectrophotometer.
The NanoDrop® ND-1000 UV-Vis Spectrophotometer is designed for measuring
small volumes. It can accurately and reproducibly measure as low as 1ul
of sample, perfect for limited sample volumes. It has a large dynamic
range eliminating the need for dilutions.
A key feature of the NanoDrop is the sample retention system, which allows
for the measurement of 1ul sample without the need for cuvettes or capillaries.
The NanoDrop’s fiber optic technology allows the user to simply wipe the
pedestal clean after making a measurement. The highly polished surfaces
of the sample retention system are resistant to sample adherence preventing
sample carryover in successive measurements. This technology also makes it
possible to recover the sample after a measurement has been completed.
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The use of the NanoDrop is free of charge. Please provide your
own lab wipes and pipet tips when using the instrument.
Appointments may be reserved by Pennington employees using the Outlook calendar.
The NanoDrop is listed in Outlook as R_GCF_NanoDrop.
Detailed instructions for using the Outlook calendar to schedule an instrument (resource) may be found in the Scheduling Resources - Frequently Asked Questions.
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The Genomics Core Facility does not charge for use of this instrument.
Please provide your own lab wipes and pipet tips when using the instrument.
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| Measurement Range: |
| Min = | 2 ng/µl |
| Max = | 3000 ng/µl (RNA) |
| 3700 ng/µl (dsDNA) |
| 2400 ng/µl (ssDNA) |
Wavelength-dependent
extinction coefficients: |
| RNA = | 40 |
| dsDNA = | 50 |
| ssDNA = | 33 |
Quality Check:
| Primary Measure | Secondary Measure |
| 260/280 | 260/230 |
| DNA ~1.8 | DNA & RNA 1.8 - 2.2 |
| RNA ~2.0 | |
If the 260/280 ratio is appreciably lower for either DNA or RNA,
it may indicate the presence of protein, phenol or other contaminants
in the sample. See "260/280 Ratio" of the Troubleshooting section in
the user manual for more details on factors that can affect this ratio.
This value is also pH dependent; see the document
RNA Sample Requirements for Microarray Analysis
for more information on the effects of pH.
The 260/230 ratio is often higher than the respective 260/280 values.
If the ratio is appreciably lower, this may indicate the presence of
co-purified contaminants.
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