Agilent 2100 Bioanalyzer

Overview

The Genomics Core Facility offers the use of two Agilent 2100 Bioanalyzers to assist investigators in evaluation of their RNA, DNA, and protein samples.

The Agilent 2100 Bioanalyzer is a microfluidics-based platform that is used for the quantification and qualification analysis of DNA, RNA, proteins, and cells. Agilent’s 2100 Expert software provides quality and quantity digital data for up to 12 samples in 30 minutes. The results are shown in a gel-like image, electropherogram and tabular formats.

The Genomics Core provides all components of the RNA 6000 Nano kit. Contact GCF well in advance of your scheduled run to obtain reagents.

Other kits that include sample-specific reagents and chips must be ordered by the researcher for use on the bioanalyzer.

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Scheduling

An appointment must be scheduled for each chip run on the Agilent. Run time on the instrument is 30 minutes. Allow approximately one hour to set up your chip prior to your scheduled instrument run.

Both bioanalyzers may be run at the same time. It is recommended to stagger appointments for each instrument by 15 minutes to avoid setup conflicts or delays.

Appointments may be reserved by Pennington employees using the Outlook calendar. The bioanalyzer instruments are listed in Outlook as R_GCF_Athena1 and R_GCF_Athena2.

Detailed instructions for using the Outlook calendar to schedule an instrument (resource) may be found in the Scheduling Resources - Frequently Asked Questions.


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Cost

  • The cost for RNA Nano 6000 reagents and run is $35.00 per run.
  • The cost for run only is $4.

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Training

For new users, the GCF will provide hands-on training for proper chip setup and use of the instrument. First time users must be trained by GCF personnel before using the instrument. Please contact GCF to schedule a training session.

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Troubleshooting

For troubleshooting help, documents are available on the desktop of the Agilent computer in the GCF. In addition, the following are available:

If you need assistance troubleshooting a run, please feel free to email your Agilent run file (denoted as a .xad file) to GCF.


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Analysis

RNA Analysis

Integrity checks and sample quantitation are essential for doing any RNA application.

The RNA 6000 Nano and RNA 6000 Pico kits are used to quantify RNA using 1 µL of a RNA sample. The 2100 bioanalyzer expert software generates the sample concentration, the RNA Integrity Number (RIN), and will calculate ribosomal ratios of the total RNA sample. The bioanalyzer software generates an electropherogram and a gel-like image for each sample. The electropherogram provides a detailed visual assessment of the quality of an RNA sample, whereas RIN can be used as a standard integrity measure that is independent of concentration, instrument or analyst.

When working with RNA, always keep the area RNase-free; use RNase-free reagents and chips to avoid sample degradation during analysis.

DNA Analysis

The DNA 1000, DNA 7500 and DNA 12000 kits allow you to automatically size and quantitate PCR fragments and restriction digests accurately and reproducibly. Only 1 µL of material is required per analysis. The bioanalyzer gives automated quantitation of each DNA fragment that is normalized against two internal markers and a ladder for sizing accuracy and reproducibility. Its broad linear dynamic range enables the detection of less abundant products and is sensitive enough for the detection of fragments down to 0.1 ng.

Protein Analysis

The Protein 80 and Protein 230 kits are capable of providing size, purity and concentration information for ten different protein samples. The Protein kits are capable of analyzing a large variety of protein samples: cell lysates, column fractions, antibodies and purified proteins. Only 4 µl of protein sample is required per analysis. The 2100 Expert software allows for both relative and absolute quantitation in one assay.


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Agilent Kits

Ordering Details

Agilent RNA 6000 Nano Kit - Part # 5067-1511
for the analysis and quantitation of total and mRNA samples

 Total RNA AssaymRNA Assay
Qualitative Range:5 - 500 ng/µl25 - 250 ng/µl
Quantitative Range:25 - 500 ng/µl25 - 250 ng/µl
Kit includes reagents, 25 chips, 2 cleaning chips, 1 syringe, reagent kit guide, ladder and markers.

Agilent RNA 6000 Pico Kit - Part # 5067-1513
for the analysis of total and mRNA samples

 Total RNA AssaymRNA Assay
Qualitative Range:200 - 5000 pg/µl500 - 5000 pg/µl
Kit includes reagents, 25 chips, 2 cleaning chips, 1 syringe, reagent kit guide, ladder and markers.

Agilent 2100 Small RNA Kit - Part # 5067-1548
for the analysis and quantitation of various RNA species, such as miRNA, siRNA, t-RNA or other oligonucleotides within given ranges

 miRNA Assay
Quantitative Range:50 - 2000 pg/µl
Qualitative Range:50 - 2000 pg/µl
Kit includes reagents, 25 chips, 1 cleaning chip, 1 syringe, reagent kit guide, ladder and markers.

Agilent DNA 1000 Kit – Part #5067-1504
for sizing and quantitation of dsDNA fragments from 25 to 1000 bp

Sizing range:25 - 1000 bp
Quantitative range:0.1 - 50 ng/µl
Kit includes reagents, 25 chips, 1 cleaning chip, 1 syringe, reagent kit guide, ladder and markers.

Agilent DNA 7500 Kit - Part # 5067-1506
for sizing and quantitation of dsDNA fragments from 100 to 7500 bp

Sizing range:100 - 7500 bp
Quantitative range:0.1 - 50 ng/µl
Kit includes reagents, 25 chips, 1 cleaning chip, 1 syringe, reagent kit guide, ladder and markers.

Agilent DNA 12000 Kit - Part # 5067-1508
for sizing and quantitation of dsDNA fragments from 100 to 12000 bp

Sizing range:100 - 12000 bp
Quantitative range:0.1 - 50 ng/µl
Kit includes reagents, 25 chips, 1 cleaning chip, 1 syringe, reagent kit guide, ladder and markers.

Agilent Protein 80 Kit - Part #5067-1515
for sizing and analysis of proteins up to 80 kDa

Sizing range:5 - 80 kDa
Quantitative range:60 - 2000 ng/µl CAII in PBS
Qualitative range:6 - 4000 ng/µl CAII and BLG
Kit includes reagents, supplies, 25 chips, 1 cleaning chip, 1 syringe, reagent kit guide, ladder and markers.

Agilent Protein 230 Kit - Part #5067-1517
for sizing and analysis of proteins up to 230 kDa

Sizing range:14 - 230 kDa
Quantitative range:15 - 2000 ng/µl CAII, 30 - 2000 ng/µl BSA in PBS
Qualitative range:6 - 5000 ng/µl CAII, 15 - 5000 ng/µl BSA in PBS
Kit includes reagents, supplies, 25 chips, 1 cleaning chip, 1 syringe, reagent kit guide, ladder and markers.

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