The Seahorse Bioscience XF-24 analyzer, which is capable of measuring the rate of
change of analytes (currently dissolved oxygen and pH) in the media surrounding
living cells, is currently available for use in the Genomics Core Facility.
The XF-24 analyzer is equipped to do analyte measurements in a 24-well format
and allows for the injection of 4 drugs per well.
The GCF will soon acquire an upgraded version of the analyzer, the XF24-3 which in
addition to measuring oxygen consumption rate (OCR) and extracellular acidification
rate (ECAR), can also measure carbon dioxide production rate (CPDR).
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The maximum amount of time per run is 3 hours (including cartridge calibration).
It is the responsibility of the user to ensure that the sensor cartridge be hydrated
overnight prior to use. The GCF has a non-CO2 incubator which can be used for
preparation of the cartridge.
Appointments for use of this instrument can be made by Pennington employees
using the Outlook calendar. The instrument resource is listed in Outlook
as R_GCF_XF24.
Detailed instructions for using the Outlook calendar to schedule an instrument (resource) may be found in the Scheduling Resources - Frequently Asked Questions.
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Training is required before use of this instrument.
Individual consultation and training may be scheduled by contacting GCF.
An XF-24 analyzer protocol is available.
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