Real-Time PCR

The Genomics Core Facility (GCF) has four Applied Biosystems 7900 Sequence Detection System instruments. The instruments may be used for gene expression, allelic discrimination (SNP analysis), and quantitative pathogen detection. The instruments are equipped to run 96 well and 384 well plates and TaqMan Low Density Arrays (TLDA).

For 384 well plate experiments, the Perkin Elmer Multiprobe II EX pipetting robot is programmed to custom pipet quantitative PCR plates. Please see Robotics for further information.

There is a $10 per run charge for use of an instrument. GCF personnel provide both informal and formal training, provide troubleshooting advice, and maintain and calibrate the instruments at no charge to the user.