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The Genomics Core Facility (GCF) is equipped with two liquid
handling robots, a MultiProbe II and a Biomek FX. The robots
are operated by GCF personnel on a fee for service basis.
The Perkin Elmer MultiProbe II EX is an eight-tip
instrument designed to pipet 2ul up to 250ul volumes into 96
and 384 well plates, Eppendorf tubes, and other labware.
This robot is used routinely to pipet 384 well RT-PCR plates
with a 10ul total reaction volume. When the cost of supplies,
reagents, and technician time is calculated, robotic pipetting
is the most accurate and cost effective answer for the pipetting
of 384 well RT-PCR plates. GCF will schedule up to six 384 well
plates for robotic pipetting in one day or the equivalent of
twenty-four 96 well plates enabling experiment data to be
available to the researcher rapidly. The MultiProbe’s
configuration gives a high degree of programming flexibility.
Custom pipetting programs can be written for special projects.
Please contact GCF personnel for project consultation well in
advance of your planned start date.
The Beckman Coulter Biomek FX is available for custom programming
of high through-put pipetting tasks. It is configured with a 96
tip head which will make pipetting transfers and liquid additions
to and from 96 and 384 well formatted plates. It is equipped
with a vacuum station which may be used for protocols requiring
liquid drawn through a column as commonly found in many purification
protocols.
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