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| About Us |
The Genomics Core Facility (GCF) provides DNA sequencing, DNA fragment
analysis, qualitative and quantitative analysis of DNA, protein, and RNA
samples, quantitative PCR, microarray RNA labeling, spotting, hybridization,
and scanning, robotics, and bioinformatics services. Individual and small
group training and consultation services are offered for sequence analysis,
real-time PCR, and microarray analysis.
The GCF laboratory services are available to any researcher. The goal of the
GCF is to provide high quality service at low cost to facilitate research.
Use of the Genomics Core Facility services are scheduled on a first come,
first serve basis. The wait time for instrument use is rarely more than one
day and is frequently shorter.
The core laboratory is approximately 900 square feet. Two ancillary laboratories
of approximately 168 square feet each house microarray hybridization, scanning,
and microarray spotting. Laboratories are supplied with emergency power with
dedicated circuits for instrumentation as well as ultrapure water, gas, air,
and vacuum supplies.
The laboratory is equipped with:
- Two Applied Biosystems 3130XL sixteen capillary genetic analyzers.
These instruments perform
DNA sequencing
and fragment analysis . They will
accommodate 96 well and 384 well format sample plates.
- Four Applied Biosystems 7900HT Sequence Detection Systems equipped with 96 well,
384 well, and low density array blocks are available for
quantitative PCR.
- An Applied Biosystems 1700 microarray system and an Illumina microarray system
are available for researchers who wish to use a commercial
microarray system.
The microarray facility has the capability to produce custom oligonucleotide
or cDNA microarrays. Slides are printed with a GeneMachine OmniGrid Microarrayer
equipped with server arm and scanned using a Perkin-Elmer ScanArray 5000.
Spotfire software and Bioconductor R Scripts are used for analysis of microarray data.
- Two pipetting robots, a Becton Dickinson Biomek FX and a Perkin Elmer MultiProbe II,
are available for
robotic liquid handling. These instruments facilitate
high-throughput pipetting of 384 well format plates. They can be programmed
for large pipetting projects.
- A Qiagen Qiacube robot will process up to
twelve samples using Qiagen Spin column kits for sample isolation and purification.
- Two Agilent 2100 Bioanalyzers are used
for protein, DNA, and RNA analysis and quantitation.
- A NanoDrop Spectrophotometer is used
for RNA and DNA quantification.
- An Odyssey Infrared Imager uses direct
infrared fluorescence detection for western blot analysis.
- For large DNA extraction and purification projects, a
Qiagen AutoPure robot is used.
- Three computer workstations are available for sequence analysis and alignment,
PCR primer design, and RT-PCR data analysis.
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